1146-1151 Zhang LAJP 3201:Zhang

The aim of this study was to optimize the preparation process of baicalin-β-cyclodextrin inclusion compound (baicalin-β-CD) by adopting Response Surface Methodology (RSM). On the basis of the single-factor experiment, the conditions of the inclusion process were optimized by adopting RSM with the factors to be investigated (i.e. the proportion of β-CD and baicalin, inclusion time and inclusion temperature); the quadratic regression equation model of the inclusion rate of baicalin-β-CD was constructed via simulation. The analysis of the experimental results showed that the optimal inclusion conditions are: proportion of β-CD and baicalin of 2.51, inclusion time of 1.57 h, and inclusion temperature of 63.79 °C. There was a good compliance between the measured result of the inclusion rate (89.83%) and the predictive value (90.22%) of the equation obtained by fitting response surface. The preparation process of baicalin-β-cyclodextrin inclusion compound established by using RSM has a higher inclusion rate, and there is a good coincidence between the regression equation and the actual situation.